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reactive oxygen species ros assay kit  (Beijing Solarbio Science)


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    Beijing Solarbio Science reactive oxygen species ros assay kit
    Reactive Oxygen Species Ros Assay Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 97/100, based on 1377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ros+assay+kit/Reactive+Oxygen+Species+Assay+Kit/pm42902504-96-15-24
    Average 97 stars, based on 1377 article reviews
    reactive oxygen species ros assay kit - by Bioz Stars, 2026-11
    97/100 stars

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    ROS Assay:

    Article Title: Yangxue Qingnao Wan ameliorates cognitive impairment in scopolamine-induced AD mice via modulating neuropeptide signaling pathways and suppressing neuroinflammation.
    Article Snippet: Background: Alzheimer’s disease (AD), a fatal neurodegenerative disorder with increasing incidence worldwide, remains a significant therapeutic challenge.. Yangxue Qingnao Wan (YXQNW), a traditional Chinese medicine, has shown promise in AD treatment.. However, its intricate mechanism of action necessitates further investigation.

    Article Title: YTHDF2 drives malignant progression in cutaneous melanoma through m⁶A-dependent HMOX1-mediated ferroptosis.
    Article Snippet: 1 Department of Plastic Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, China 2 Department of Plastic Surgery, Central Hospital Affiliated to Shandong First Medical University, Jinan 250013, Shandong, China 3 Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, Shandong, China Abstract N6-methyladenosine (m6A) modification plays a critical role in eukaryotic mRNA and is significantly associated with cutaneous melanoma.. This study investigated the role of the m6A-binding protein YTHDF2 in the pathogenesis of cutaneous melanoma and its potential therapeutic applications.. Analyses of public databases indicated that YTHDF2 exhibited elevated expression in various cancers, including cutaneous melanoma, where its levels positively correlated with PD-L1 and negatively correlated with immune and stromal scores.

    Article Title: Quorum sensing orchestrates salt adaptation of anammox communities: synergistic electron transfer and metabolic cross-feeding mechanisms
    Article Snippet: Anaerobic ammonium oxidation (anammox) technology has demonstrated excellent potential in treating nitrogen-rich saline wastewater, but fluctuations in salinity often lead to instability in nitrogen removal efficiency.. While prior research focused on anammox bacteria (AnAOB) salt tolerance, the role of microbial community synergy remains understudied.. This study innovatively employed quorum sensing (QS) regulation, using signal molecule N-hexanoyl-L-homoserine lactone (C6-HSL) and inhibitor vanillin, to investigate their effects on the system's salinity adaptability.

    Article Title: LncRNA EGFR-AS1 inhibits ferroptosis to reduce radiosensitivity of cervical cancer through the m6A/IGF2BP3/APAF1 axis.
    Article Snippet: .. Reactive Oxygen Species (ROS) level measurement 268 We measured the level of ROS by the ROS Assay Kit (CA1410, 269 Solarbio). .. Detection of malondialdehyde (MDA) 264 We measured the levels of MDA in cervical cancer cells using MDA 265 Assay Kit (S0131S, Beyotime, China).

    Article Title: Astragaloside IV Alleviates Trueperella pyogenes -Induced Endometritis via the Nrf2/HO-1 Signaling Pathway
    Article Snippet: Malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity were measured using commercial assay kits (Solarbio, Beijing, China) by determining absorbance at 532/600 nm and 450 nm, respectively, with calculations performed according to the manufacturer’s instructions. .. Intracellular reactive oxygen species (ROS) levels were assessed using a corresponding ROS assay kit (Solarbio, Beijing, China). ..

    Article Title: Bmced6 enhances BmNPV infection in BmN cells by modulating autophagy and mitochondrial homeostasis.
    Article Snippet: Autophagy is a conserved cellular process with dual roles in antiviral defense and viral utilization that plays a crucial role in host-pathogen interactions.. Here, we investigated the function and related mechanisms of Bmced6 in domestic silkworm, Bombyx mori, during the infestation of B. mori nucleopolyhedrovirus (BmNPV).. At first, immunofluorescence indicated that Bmced6 was mainly localized in the cytoplasm.

    Article Title: Astragaloside IV Alleviates Trueperella pyogenes -Induced Endometritis via the Nrf2/HO-1 Signaling Pathway.
    Article Snippet: Malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity were measured using commercial assay kits (Solarbio, Beijing, China) by determining absorbance at 532/600 nm and 450 nm, respectively, with calculations performed according to the manufacturer’s instructions. .. Intracellular reactive oxygen species (ROS) levels were assessed using a corresponding ROS assay kit (Solarbio, Beijing, China). ..

    Flow Cytometry:

    Article Title: YTHDF2 drives malignant progression in cutaneous melanoma through m⁶A-dependent HMOX1-mediated ferroptosis.
    Article Snippet: 1 Department of Plastic Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, China 2 Department of Plastic Surgery, Central Hospital Affiliated to Shandong First Medical University, Jinan 250013, Shandong, China 3 Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, Shandong, China Abstract N6-methyladenosine (m6A) modification plays a critical role in eukaryotic mRNA and is significantly associated with cutaneous melanoma.. This study investigated the role of the m6A-binding protein YTHDF2 in the pathogenesis of cutaneous melanoma and its potential therapeutic applications.. Analyses of public databases indicated that YTHDF2 exhibited elevated expression in various cancers, including cutaneous melanoma, where its levels positively correlated with PD-L1 and negatively correlated with immune and stromal scores.

    other:

    Article Title: Dietary quercetin enhances T cell immunity and disease resistance in tilapia via modulating antioxidant and inflammatory responses
    Article Snippet: The increasing intensity of aquaculture production has exposed farmed fish to persistent pathogen challenges and oxidative stress, highlighting the urgent need for safe and effective nutritional immunostimulants.. Quercetin, a natural polyphenolic compound, is known for its antioxidant and anti-inflammatory properties; however, its regulatory effects on fish T-cell immunity remain poorly defined.. In this study, we investigated the immunomodulatory role of dietary quercetin in Nile tilapia (Oreochromis niloticus), with a particular focus on Tcell–mediated immunity.



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    ERK inhibition attenuates TXNRD1-driven SPP1-high/CXCL9-low remodeling in EGCs (A) CCK-8 viability assay of cultured rat EGCs treated with the indicated concentrations of SCH772984 (0–40 nM) for 24 h; for (B)–(G), cultured EGCs were assigned to Control+DMSO, LV-OE-NC+DMSO, LV-OE-TXNRD1+DMSO, and LV-OE-TXNRD1+SCH772984 groups. SCH772984 was used at 10 nM. (B and C) Representative WB of TXNRD1, p-Raf, Raf, p-MEK, MEK, p-ERK, ERK, and GAPDH (B) and densitometric quantification (C). (D and E) Representative FCM histograms of intracellular <t>ROS</t> (D) and quantification of relative ROS fluorescence intensity (E). (F and G) Representative IF images of CXCL9 and SPP1 in cultured EGCs (F) and quantification of relative fluorescence intensity (G). n = 3 independent biological experiments; each dot represents one independently performed experiment. Quantitative data are presented as mean ± SD with individual data points. One-way ANOVA followed by Tukey’s multiple-comparison test was used. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, not significant. Blots, FCM plots, and micrographs are representative of three independent experiments. Scale bars, 100 μm in (F).
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    reactive oxygen species ros assay kit  (Beijing Solarbio Science)
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    Beijing Solarbio Science reactive oxygen species ros assay kit
    ERK inhibition attenuates TXNRD1-driven SPP1-high/CXCL9-low remodeling in EGCs (A) CCK-8 viability assay of cultured rat EGCs treated with the indicated concentrations of SCH772984 (0–40 nM) for 24 h; for (B)–(G), cultured EGCs were assigned to Control+DMSO, LV-OE-NC+DMSO, LV-OE-TXNRD1+DMSO, and LV-OE-TXNRD1+SCH772984 groups. SCH772984 was used at 10 nM. (B and C) Representative WB of TXNRD1, p-Raf, Raf, p-MEK, MEK, p-ERK, ERK, and GAPDH (B) and densitometric quantification (C). (D and E) Representative FCM histograms of intracellular <t>ROS</t> (D) and quantification of relative ROS fluorescence intensity (E). (F and G) Representative IF images of CXCL9 and SPP1 in cultured EGCs (F) and quantification of relative fluorescence intensity (G). n = 3 independent biological experiments; each dot represents one independently performed experiment. Quantitative data are presented as mean ± SD with individual data points. One-way ANOVA followed by Tukey’s multiple-comparison test was used. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, not significant. Blots, FCM plots, and micrographs are representative of three independent experiments. Scale bars, 100 μm in (F).
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    ERK inhibition attenuates TXNRD1-driven SPP1-high/CXCL9-low remodeling in EGCs (A) CCK-8 viability assay of cultured rat EGCs treated with the indicated concentrations of SCH772984 (0–40 nM) for 24 h; for (B)–(G), cultured EGCs were assigned to Control+DMSO, LV-OE-NC+DMSO, LV-OE-TXNRD1+DMSO, and LV-OE-TXNRD1+SCH772984 groups. SCH772984 was used at 10 nM. (B and C) Representative WB of TXNRD1, p-Raf, Raf, p-MEK, MEK, p-ERK, ERK, and GAPDH (B) and densitometric quantification (C). (D and E) Representative FCM histograms of intracellular <t>ROS</t> (D) and quantification of relative ROS fluorescence intensity (E). (F and G) Representative IF images of CXCL9 and SPP1 in cultured EGCs (F) and quantification of relative fluorescence intensity (G). n = 3 independent biological experiments; each dot represents one independently performed experiment. Quantitative data are presented as mean ± SD with individual data points. One-way ANOVA followed by Tukey’s multiple-comparison test was used. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, not significant. Blots, FCM plots, and micrographs are representative of three independent experiments. Scale bars, 100 μm in (F).
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    ERK inhibition attenuates TXNRD1-driven SPP1-high/CXCL9-low remodeling in EGCs (A) CCK-8 viability assay of cultured rat EGCs treated with the indicated concentrations of SCH772984 (0–40 nM) for 24 h; for (B)–(G), cultured EGCs were assigned to Control+DMSO, LV-OE-NC+DMSO, LV-OE-TXNRD1+DMSO, and LV-OE-TXNRD1+SCH772984 groups. SCH772984 was used at 10 nM. (B and C) Representative WB of TXNRD1, p-Raf, Raf, p-MEK, MEK, p-ERK, ERK, and GAPDH (B) and densitometric quantification (C). (D and E) Representative FCM histograms of intracellular <t>ROS</t> (D) and quantification of relative ROS fluorescence intensity (E). (F and G) Representative IF images of CXCL9 and SPP1 in cultured EGCs (F) and quantification of relative fluorescence intensity (G). n = 3 independent biological experiments; each dot represents one independently performed experiment. Quantitative data are presented as mean ± SD with individual data points. One-way ANOVA followed by Tukey’s multiple-comparison test was used. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, not significant. Blots, FCM plots, and micrographs are representative of three independent experiments. Scale bars, 100 μm in (F).
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    ERK inhibition attenuates TXNRD1-driven SPP1-high/CXCL9-low remodeling in EGCs (A) CCK-8 viability assay of cultured rat EGCs treated with the indicated concentrations of SCH772984 (0–40 nM) for 24 h; for (B)–(G), cultured EGCs were assigned to Control+DMSO, LV-OE-NC+DMSO, LV-OE-TXNRD1+DMSO, and LV-OE-TXNRD1+SCH772984 groups. SCH772984 was used at 10 nM. (B and C) Representative WB of TXNRD1, p-Raf, Raf, p-MEK, MEK, p-ERK, ERK, and GAPDH (B) and densitometric quantification (C). (D and E) Representative FCM histograms of intracellular <t>ROS</t> (D) and quantification of relative ROS fluorescence intensity (E). (F and G) Representative IF images of CXCL9 and SPP1 in cultured EGCs (F) and quantification of relative fluorescence intensity (G). n = 3 independent biological experiments; each dot represents one independently performed experiment. Quantitative data are presented as mean ± SD with individual data points. One-way ANOVA followed by Tukey’s multiple-comparison test was used. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, not significant. Blots, FCM plots, and micrographs are representative of three independent experiments. Scale bars, 100 μm in (F).
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    ERK inhibition attenuates TXNRD1-driven SPP1-high/CXCL9-low remodeling in EGCs (A) CCK-8 viability assay of cultured rat EGCs treated with the indicated concentrations of SCH772984 (0–40 nM) for 24 h; for (B)–(G), cultured EGCs were assigned to Control+DMSO, LV-OE-NC+DMSO, LV-OE-TXNRD1+DMSO, and LV-OE-TXNRD1+SCH772984 groups. SCH772984 was used at 10 nM. (B and C) Representative WB of TXNRD1, p-Raf, Raf, p-MEK, MEK, p-ERK, ERK, and GAPDH (B) and densitometric quantification (C). (D and E) Representative FCM histograms of intracellular <t>ROS</t> (D) and quantification of relative ROS fluorescence intensity (E). (F and G) Representative IF images of CXCL9 and SPP1 in cultured EGCs (F) and quantification of relative fluorescence intensity (G). n = 3 independent biological experiments; each dot represents one independently performed experiment. Quantitative data are presented as mean ± SD with individual data points. One-way ANOVA followed by Tukey’s multiple-comparison test was used. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, not significant. Blots, FCM plots, and micrographs are representative of three independent experiments. Scale bars, 100 μm in (F).
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    ERK inhibition attenuates TXNRD1-driven SPP1-high/CXCL9-low remodeling in EGCs (A) CCK-8 viability assay of cultured rat EGCs treated with the indicated concentrations of SCH772984 (0–40 nM) for 24 h; for (B)–(G), cultured EGCs were assigned to Control+DMSO, LV-OE-NC+DMSO, LV-OE-TXNRD1+DMSO, and LV-OE-TXNRD1+SCH772984 groups. SCH772984 was used at 10 nM. (B and C) Representative WB of TXNRD1, p-Raf, Raf, p-MEK, MEK, p-ERK, ERK, and GAPDH (B) and densitometric quantification (C). (D and E) Representative FCM histograms of intracellular <t>ROS</t> (D) and quantification of relative ROS fluorescence intensity (E). (F and G) Representative IF images of CXCL9 and SPP1 in cultured EGCs (F) and quantification of relative fluorescence intensity (G). n = 3 independent biological experiments; each dot represents one independently performed experiment. Quantitative data are presented as mean ± SD with individual data points. One-way ANOVA followed by Tukey’s multiple-comparison test was used. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, not significant. Blots, FCM plots, and micrographs are representative of three independent experiments. Scale bars, 100 μm in (F).
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    ERK inhibition attenuates TXNRD1-driven SPP1-high/CXCL9-low remodeling in EGCs (A) CCK-8 viability assay of cultured rat EGCs treated with the indicated concentrations of SCH772984 (0–40 nM) for 24 h; for (B)–(G), cultured EGCs were assigned to Control+DMSO, LV-OE-NC+DMSO, LV-OE-TXNRD1+DMSO, and LV-OE-TXNRD1+SCH772984 groups. SCH772984 was used at 10 nM. (B and C) Representative WB of TXNRD1, p-Raf, Raf, p-MEK, MEK, p-ERK, ERK, and GAPDH (B) and densitometric quantification (C). (D and E) Representative FCM histograms of intracellular <t>ROS</t> (D) and quantification of relative ROS fluorescence intensity (E). (F and G) Representative IF images of CXCL9 and SPP1 in cultured EGCs (F) and quantification of relative fluorescence intensity (G). n = 3 independent biological experiments; each dot represents one independently performed experiment. Quantitative data are presented as mean ± SD with individual data points. One-way ANOVA followed by Tukey’s multiple-comparison test was used. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, not significant. Blots, FCM plots, and micrographs are representative of three independent experiments. Scale bars, 100 μm in (F).
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    Image Search Results


    ERK inhibition attenuates TXNRD1-driven SPP1-high/CXCL9-low remodeling in EGCs (A) CCK-8 viability assay of cultured rat EGCs treated with the indicated concentrations of SCH772984 (0–40 nM) for 24 h; for (B)–(G), cultured EGCs were assigned to Control+DMSO, LV-OE-NC+DMSO, LV-OE-TXNRD1+DMSO, and LV-OE-TXNRD1+SCH772984 groups. SCH772984 was used at 10 nM. (B and C) Representative WB of TXNRD1, p-Raf, Raf, p-MEK, MEK, p-ERK, ERK, and GAPDH (B) and densitometric quantification (C). (D and E) Representative FCM histograms of intracellular ROS (D) and quantification of relative ROS fluorescence intensity (E). (F and G) Representative IF images of CXCL9 and SPP1 in cultured EGCs (F) and quantification of relative fluorescence intensity (G). n = 3 independent biological experiments; each dot represents one independently performed experiment. Quantitative data are presented as mean ± SD with individual data points. One-way ANOVA followed by Tukey’s multiple-comparison test was used. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, not significant. Blots, FCM plots, and micrographs are representative of three independent experiments. Scale bars, 100 μm in (F).

    Journal: iScience

    Article Title: TXNRD1-driven enteric glial remodeling promotes neuronal dysfunction in Hirschsprung disease

    doi: 10.1016/j.isci.2026.117636

    Figure Lengend Snippet: ERK inhibition attenuates TXNRD1-driven SPP1-high/CXCL9-low remodeling in EGCs (A) CCK-8 viability assay of cultured rat EGCs treated with the indicated concentrations of SCH772984 (0–40 nM) for 24 h; for (B)–(G), cultured EGCs were assigned to Control+DMSO, LV-OE-NC+DMSO, LV-OE-TXNRD1+DMSO, and LV-OE-TXNRD1+SCH772984 groups. SCH772984 was used at 10 nM. (B and C) Representative WB of TXNRD1, p-Raf, Raf, p-MEK, MEK, p-ERK, ERK, and GAPDH (B) and densitometric quantification (C). (D and E) Representative FCM histograms of intracellular ROS (D) and quantification of relative ROS fluorescence intensity (E). (F and G) Representative IF images of CXCL9 and SPP1 in cultured EGCs (F) and quantification of relative fluorescence intensity (G). n = 3 independent biological experiments; each dot represents one independently performed experiment. Quantitative data are presented as mean ± SD with individual data points. One-way ANOVA followed by Tukey’s multiple-comparison test was used. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, not significant. Blots, FCM plots, and micrographs are representative of three independent experiments. Scale bars, 100 μm in (F).

    Article Snippet: ROS assay kit , MedChemExpress , HY-K0320.

    Techniques: Inhibition, CCK-8 Assay, Viability Assay, Cell Culture, Control, Fluorescence, Comparison

    NAC suppresses TXNRD1-induced ROS accumulation, ERK activation, and SPP1-high/CXCL9-low remodeling in EGCs Cultured rat EGCs were assigned to Control+DMSO, LV-OE-NC+DMSO, LV-OE-TXNRD1+DMSO, LV-OE-NC+NAC, and LV-OE-TXNRD1+NAC groups. NAC was used at 1. (A and B) Representative WB of p-ERK, ERK, and tubulin (A) and quantification of the p-ERK/ERK ratio (B). (C and D) Representative FCM histograms of intracellular ROS (C) and quantification of relative ROS fluorescence intensity (D). (E) TrxR activity in EGC lysates. (F and G) Representative IF images of CXCL9 and SPP1 in cultured EGCs (F) and quantification of relative fluorescence intensity (G). n = 3 independent biological experiments; each dot represents one independently performed experiment. Quantitative data are presented as mean ± SD with individual data points. One-way ANOVA followed by Tukey’s multiple-comparison test was used. ∗ p < 0.05, ∗∗∗ p < 0.001; ns, not significant. Blots, FCM plots, and micrographs are representative of three independent experiments. Scale bars, 100 μm in (F).

    Journal: iScience

    Article Title: TXNRD1-driven enteric glial remodeling promotes neuronal dysfunction in Hirschsprung disease

    doi: 10.1016/j.isci.2026.117636

    Figure Lengend Snippet: NAC suppresses TXNRD1-induced ROS accumulation, ERK activation, and SPP1-high/CXCL9-low remodeling in EGCs Cultured rat EGCs were assigned to Control+DMSO, LV-OE-NC+DMSO, LV-OE-TXNRD1+DMSO, LV-OE-NC+NAC, and LV-OE-TXNRD1+NAC groups. NAC was used at 1. (A and B) Representative WB of p-ERK, ERK, and tubulin (A) and quantification of the p-ERK/ERK ratio (B). (C and D) Representative FCM histograms of intracellular ROS (C) and quantification of relative ROS fluorescence intensity (D). (E) TrxR activity in EGC lysates. (F and G) Representative IF images of CXCL9 and SPP1 in cultured EGCs (F) and quantification of relative fluorescence intensity (G). n = 3 independent biological experiments; each dot represents one independently performed experiment. Quantitative data are presented as mean ± SD with individual data points. One-way ANOVA followed by Tukey’s multiple-comparison test was used. ∗ p < 0.05, ∗∗∗ p < 0.001; ns, not significant. Blots, FCM plots, and micrographs are representative of three independent experiments. Scale bars, 100 μm in (F).

    Article Snippet: ROS assay kit , MedChemExpress , HY-K0320.

    Techniques: Activation Assay, Cell Culture, Control, Fluorescence, Activity Assay, Comparison

    Journal: iScience

    Article Title: TXNRD1-driven enteric glial remodeling promotes neuronal dysfunction in Hirschsprung disease

    doi: 10.1016/j.isci.2026.117636

    Figure Lengend Snippet:

    Article Snippet: ROS assay kit , MedChemExpress , HY-K0320.

    Techniques: Model Organisms, Recombinant, CCK-8 Assay, Apoptosis Assay, ROS Assay, Calcium Assay, Enzyme-linked Immunosorbent Assay, Generated, Software, Genotyping